Enable JavaScript to visit this website.

CYTOLOGY

  • For cellblocks, the same IHC protocols can be used as for formalin-fixed paraffin-embedded (FFPE) tissue specimens.
  • ROS1 IHC (using the D4D6 mAb) on the BondMax immunostainer can be used on conventional, ethanol-fixed and Papanicolaou stained cytological specimens, including smears or cytospin preparations

Bubendorf L et al. Virchows Arch. 2016;469:489–503

IHC BondMax protocol

LEICA BOND-MAX: BOND Polymer Refine
Detection Kit with AEC

Cell Conditioning:
HIER
ER2: 10 minutes (Epitope Retrieval
solution 2) 80°C
Ab incubation: 30 minutes 20-25°C
Amplification:
Included in kit
Post Primary 15 minutes 20-25°C
Polymer 15 minutes 20-25°C
Counterstain [Hematoxylin] 5 minutes 20-25°C

 

LEICA BOND-MAX: BOND Polymer Refine Detection Kit with
AEC for Cytology smears

  1. Staining: AEC5
  2. No Deparaffinization for Cytology
  3. Cell Conditioning: HIER: ER2
  4. EPITOPE RETRIEVAL 2: 10 minutes 80°C
  5. PEROXIDE BLOCK: 5 minutes 20-25°C
  6. PRIMARY ANTIBODY ROS1 CELL SIGNALING (clone D4D6) 1/200
  7. Apply primary antibody and incubate for 30 minutes 20-25°C
  8. POST PRIMARY incubate for 15 minutes 20-25°C
  9. POLYMER incubate for 15 minutes 20-25°C
  10. CHROMOGEN AEC incubate for 10 minutes 20-25°C
  11. Counterstain HEMATOXYLIN
  12. Apply one drop of HEMATOXYLIN [in Kit included] and incubate for 5 minutes 20-25°C

(*HIER: Heat Induced Epitope Retrieval)