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ROS1 Fluorescence in situ hybridisation (FISH)

Commercially available DNA FISH probes for testing ROS1 rearrangements

Manufacturer Analyte Catalog No. Genomic Position Size (~Kb)
Orientation 3’ (Green)        
ZytoVision ZytoLight SPEC ROS1 Dual Color Break Apart Probe* Z-2144 Ensembl GRCh37/hg19
116,912,298-117,255
715
Leica Biosystems/Kreatech ROS1 (6q22) Proximal-XL Probe 06Q006V495 Undisclosed 450
Cytocell ROS1-GOPC (FIG) Proximal Probe LPS-510-A Ensembl GRCh37/hg19
117,037,649 117,535,48
498
Agilent Technologies/Dako SureFISH ROS1 3’Break-Apart Probe G100952G UCSC GRCh37/hg19
116,510,648 117,642,558
1,132
Abbott Molecular Vysis LSI ROS1 (Cen) SpectrumGreen Probe 08N07-020 UCSC GRCh37/hg19
117,177,382 117,733,849
557
Orientation 5’ (Red)        
Zytovision ZytoLight SPEC ROS1 Dual Color Break Apart Probe* Z-2144 Ensembl GRCh37/hg19
117,659,135-117,871,701
215
Leica Biosystems/Kreatech ROS1 (6q22) Distal-XL Probe 06Q007V550 Undisclosed 260
Cytocell ROS1-GOPC (FIG) Distal Probe LPS 511-A Ensembl GRCh37/hg19
117,665,515 117,871,721
206
Agilent Techologies/Dako SureFISH ROS1 5’Break-Apart Probe G100953R UCSC GRCh37/hg19
117,642,558 117,888,013
245
Abbott Molecular Vysis LSI ROS1 (Tel) SpectrumOrange Probe 08N05-020 UCSC GRCh37/hg19
117,761,413 118,078,260
317

FISH, fluorescence in situ hybridisation


IASLC Atlas of ALK and ROS1 Testing in Lung Cancer, IASLC, Colorado, USA